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KMID : 1094720190240010240
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Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 1 p.240 ~ p.249
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Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification
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Jin Hyeon-Su
Park Seon-Yeong
Kim Ji-Yeon
Lee Jae-Eun
Lee Han-Seung
Kang Nam-Joo
Lee Dong-Woo
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Abstract
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The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.
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KEYWORD
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Fervidobacterium islandicum, LMW keratin peptide, anaerobic digestion, large-scale purification, skin anti-aging
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